Research Topic · Peer-Reviewed

Horseradish Peroxidase

Horseradish peroxidase (HRP) is a heme-containing oxidoreductase enzyme, isolated from the roots of horseradish, that catalyzes the oxidation of a wide range of organic substrates using hydrogen peroxide as the electron acceptor. Its catalytic versatility, stability, and ability to generate colored, chemiluminescent…

Curated from this journal's research 📚 12 peer-reviewed articles cited Cited 84× across the literature 🔖 ISSN 3070-5657 🗓 Reviewed July 2026

Overview

Horseradish peroxidase (HRP) is a heme-containing oxidoreductase enzyme, isolated from the roots of horseradish, that catalyzes the oxidation of a wide range of organic substrates using hydrogen peroxide as the electron acceptor. Its catalytic versatility, stability, and ability to generate colored, chemiluminescent, or fluorescent products from chromogenic substrates make it one of the most widely used reporter enzymes in the life sciences. HRP is conjugated to antibodies and other detection reagents to enable sensitive, quantifiable signal generation, and it is a workhorse of immunohistochemistry, western blotting, and the enzyme-linked immunosorbent assay (ELISA), where enzyme amplification permits detection of low-abundance proteins and antigens. As a classical neuroanatomical tracer, HRP has also been used to map neuronal connectivity. Its mechanism proceeds through high-valent oxoferryl intermediates that sequentially oxidize substrate molecules, and it is exploited in biosensors and analytical chemistry beyond research labeling. Work on the evolution and application of enzyme immunoassays and ELISA illustrates the broader detection platforms in which peroxidase-based reporters operate. The journal publishes peer-reviewed research in molecular and cellular biology and biochemistry, including the antibody-based and enzymatic detection methods, such as immunoassays and immunoblotting, in which horseradish peroxidase and related reporter enzymes are central analytical tools.

Research published in this journal

12 peer-reviewed articles, ranked by relevance. Each links to its DOI.

How this research is being cited

The 12 articles above have been cited 84 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.

A sample of recent works citing this journal's research on Horseradish Peroxidase, linking to each citing work.

Editorial oversight

Curated from peer-reviewed research published in Embryology (ISSN 3070-5657).

Journal editorial board
Kei-ichiro Ishiguro · Japan Colin Ockleford · United Kingdom Thimios Mitsiadis · Switzerland

This page summarises published research for orientation; it is not medical or professional advice.